Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.
نویسندگان
چکیده
The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 332 شماره
صفحات -
تاریخ انتشار 2006